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Photo Dec 9, 2012, 12-53 AM
Comments (25)
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    1) DMSO is just a solvent and is not involved in the polymerization
    2) the anhydride hydrolyzes to yield 2 acids which are anionic
    3) targeting drugs against transcription factors is very difficult. Their activation domains are disordered and bind multiple often generic surfaces, while the DNA binding domain can’t be inhibited selectively over most other DNA binding proteins. Most examples of ligand inducible/repressable transcription factors have an additional domain naturally and are usually found only in regulated processes unlikely sperm production which on 100% of the time following puberty. It may be possible but genome modifying approaches are likely easier.
    However genome editing/silencing is still far out and fertility is at the bottom of the priority list. Reversible silencing requires constant treatment with a cas9 (the best editing technology) repressor fusion, or perhaps the permanent insert of a ligand inducible cassette. However modification efficiencies can low and vary greatly across cell types even after controlling for transduction efficiency. This issue of efficiency is not unique. Most genetic techniques have similar issues however with cells lines/yeast you can simplify select for successful events, which is not the case in in vivo. Also cas9 has some serious issues with offsite activity which causes translocations which could potential lead to cancer or disregulatin of other processes. Finally there was recently an meeting of prominent scientist recommending a slow do of genome editing research to ensure ethical implementation(1). Which is going to slow things down immensely. Just though I’d fill you in the issues associated with several of the approaches you mentioned and my opinion
    1. Science 20 March 2015: 1301.

  5. aaa says:

    those acids from the anhydride probably chelate a metal (Mg or Zn) of some sort to facilitate adsorption of cell membranes

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